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SÉPARATION CELLULAIRE : UNE APPROCHE PRATIQUE (APPROCHE PRATIQUE par Derek Fisher * comme neuf*

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Numéro de l'objet eBay :335341968221

Caractéristiques de l'objet

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ISBN-10: 019963579X

Book Title: Cell Separation: A Practical Approach (Practical Approach Series)

ISBN: 9780199635795

Publication Name: Cell Separation : a Practical Approach

Item Length: 9.2in

Publisher: Oxford University Press, Incorporated

Series: Practical Approach Ser.

Publication Year: 1999

Type: Textbook

Format: Uk-Trade Paper

Language: English

Item Height: 0.6in

Author: Gillian E. Francis

Item Width: 6.1in

Item Weight: 18 Oz

Number of Pages: 286 Pages

À propos de ce produit

Product Information

The successful separation of specific cells requires not only a knowledge of potential methods, but also detailed protocols that are known to work well in practice. This book is unique in that it brings together the best methods currently used for separating cells. For each approach, the authors provide clear explanations of the theoretical basis of the procedures as well as practical details of the technique. The first chapter describes the isolation of single cell suspensions from animal tissues and from adherent cell culture. It includes the use of lavages, problems of extracellular matrix, methods of assessing cell viability, and the separation of viable from non-viable cells. The following seven chapters discuss specific methods for the separation of cells and the fractionation of cell populations. Sedimentation methods, centrifugal elutriation, partitioning in aqueous two-phase systems, flow cytometry, as well as numerous other procedures are discusses as well. This book should be read by all researchers for whom cell separation is the starting point for their experimental work. As such, it is an invaluable laboratory manual for cell biologists, biochemists, molecular biologists, and physiologists.

Product Identifiers

Publisher

Oxford University Press, Incorporated

ISBN-10

019963579x

ISBN-13

9780199635795

eBay Product ID (ePID)

20038266346

Product Key Features

Author

Gillian E. Francis

Publication Name

Cell Separation : a Practical Approach

Format

Uk-Trade Paper

Language

English

Series

Practical Approach Ser.

Publication Year

1999

Type

Textbook

Number of Pages

286 Pages

Dimensions

Item Length

9.2in

Item Height

0.6in

Item Width

6.1in

Item Weight

18 Oz

Additional Product Features

Series Volume Number

193

Reviews

''...the book is a perfect introduction manual for all laboratory workers in the life sciences.''Aslib Book Guide, Vol.64, no.3, March 1999.'...the book gives detailed and reliable descriptions of most available methods for preparation and separation of isolated cells from tissues. It should certainly find its place in laboratories that use or plan to use methods for cell separation' Cell Biology International Vol.24, No.6, "In this reference British researchers have contributed articles covering the process of cell separation using a number of different methods, with explanations of the theoretical bases for these procedures. The chapters explain when a particular method would be best used, based on cell size, density, surface charge, hydrophobic surface properties, and antigen status. The methods described include sedimentation, centrifugal elutriation, partitioning in aqueous two-phase systems, flow cytometry, immuno methods (including magnetic, column, and panning techniques), and free flow electrophoresis."--SciTech Book News "The title of this book describes the contents within, and reviews a subject that is underrepresented in the literature. The seven chapters discuss the preparation of single cell suspensions and six typical separation methods: sedimentation, centrifugal elutriation, partitioning in aqueous two-phase systems, flow cytometry, immunomethods (magnetic, column, and panning techniques), and free flow electrophoresis. The descriptions emphasize practical applications of these techniques including protocols for most, if not all methods. Although the theoretical basis of these techniques are not heavily emphasized, adequate references are given for those interested."--The Quarterly Review of Biology, "In this reference British researchers have contributed articles covering the process of cell separation using a number of different methods, with explanations of the theoretical bases for these procedures. The chapters explain when a particular method would be best used, based on cell size, density, surface charge, hydrophobic surface properties, and antigen status. The methods described include sedimentation, centrifugal elutriation, partitioning in aqueous two-phase systems, flow cytometry, immuno methods (including magnetic, column, and panning techniques), and free flow electrophoresis."--SciTech Book News"The title of this book describes the contents within, and reviews a subject that is underrepresented in the literature. The seven chapters discuss the preparation of single cell suspensions and six typical separation methods: sedimentation, centrifugal elutriation, partitioning in aqueous two-phase systems, flow cytometry, immunomethods (magnetic, column, and panning techniques), and free flow electrophoresis. The descriptions emphasize practical applications of these techniques including protocols for most, if not all methods. Although the theoretical basis of these techniques are not heavily emphasized, adequate references are given for those interested."--The Quarterly Review of Biology, '...the book gives detailed and reliable descriptions of most available methods for preparation and separation of isolated cells from tissues. It should certainly find its place in laboratories that use or plan to use methods for cell separation' Cell Biology International Vol.24, No.6, ''...the book is a perfect introduction manual for all laboratory workers in the life sciences.''Aslib Book Guide, Vol.64, no.3, March 1999., '...the book gives detailed and reliable descriptions of most availablemethods for preparation and separation of isolated cells from tissues. It shouldcertainly find its place in laboratories that use or plan to use methods forcell separation' Cell Biology International Vol.24, No.6, ''...the book is a perfect introduction manual for all laboratory workersin the life sciences.''Aslib Book Guide, Vol.64, no.3, March 1999., "In this reference British researchers have contributed articles covering the process of cell separation using a number of different methods, with explanations of the theoretical bases for these procedures. The chapters explain when a particular method would be best used, based on cell size, density, surface charge, hydrophobic surface properties, and antigen status. The methods described include sedimentation, centrifugal elutriation, partitioning in aqueous two-phase systems, flow cytometry, immuno methods (including magnetic, column, and panning techniques), and free flow electrophoresis."-- SciTech Book News "The title of this book describes the contents within, and reviews a subject that is underrepresented in the literature. The seven chapters discuss the preparation of single cell suspensions and six typical separation methods: sedimentation, centrifugal elutriation, partitioning in aqueous two-phase systems, flow cytometry, immunomethods (magnetic, column, and panning techniques), and free flow electrophoresis. The descriptions emphasize practical applications of these techniques including protocols for most, if not all methods. Although the theoretical basis of these techniques are not heavily emphasized, adequate references are given for those interested."-- The Quarterly Review of Biology

Table of Content

1. Preparation of single cell suspensions2. Fractionation of cells by sedimentation methods3. Centrifugal elutriationAppendix: Simulation of centrifugal elutriation for the Beckman E-6B chamber4. Separation and frustrationation of cells by partitioning in aqueous two-phase systemsAppendix: Manually operated counter current distribution apparatus5. Separation cells by flow cytometry6. Immuno methods: magnetic, columns and panning techniques7. Separation of cells using free flow electrophoresisAppendix: Suppliers

1998

Topic

Life Sciences / Cell Biology, Histology

Lccn

98-003833

Dewey Decimal

571.6028

Intended Audience

College Audience

Dewey Edition

Illustrated

Yes

Genre

Science, Medical

Description de l'objet du vendeur

Le vendeur assume l'entière responsabilité de cette annonce.

Numéro de l'objet eBay :335341968221

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