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Silençage des gènes par interférence ARN : technologie et application

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Lieu : Albuquerque, New Mexico, États-Unis
Livraison :
Livraison prévue entre le lun. 17 juin et le ven. 21 juin à 43230
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Numéro de l'objet eBay :256468099426
Dernière mise à jour : avr. 06, 2024 11:58:03 HAEAfficher toutes les modificationsAfficher toutes les modifications

Caractéristiques de l'objet

ISBN
9780849321412
Language
English
Illustrator
Yes
Author
Muhammad Sohail
Publisher
CRC Press LLC
Topic
Life Sciences / Molecular Biology, Life Sciences / Biochemistry, Life Sciences / Genetics & Genomics, Biochemistry, Science, Medical

À propos de ce produit

Product Information

Maximizing the potential of RNA interference in functional genomics - as well as in the development of therapeutics - continues to be at the forefront of biomedical research. Unlike journal articles, Gene Silencing by RNA Interference: Technology and Application combines essential background to the RNAi field with practical techniques designed by renowned researchers to provide the most diverse and in-depth examination of the subject yet. This book describes methods and protocols for gene silencing and RNA interference. Each chapter provides necessary background to the subject and then gives detailed methods in easy-to-follow steps, along with troubleshooting hints and tips. Following a general and historical introduction, chapters two through eight focus on technical details of the various methods of siRNA design, chemical and enzymatic production, plasmid- and virus-mediated intracellular expression, and other RNAi tools. Chapter nine discusses the exogenous delivery of siRNAs into cells, and the final chapters of the book detail the application of RNAi to dissect gene function in a number of biological systems, including cell-free systems, cultured cells, and whole organisms. From the design and production of RNAi tools to their applications, Gene Silencing by RNA Interference: Technology and Application is the first to present the entire spectrum of activity, design, production, and delivery of RNAi reagents, providing a welcome guide for both academic and commercial use.

Product Identifiers

Publisher
CRC Press LLC
ISBN-10
0849321417
ISBN-13
9780849321412
eBay Product ID (ePID)
30247830

Product Key Features

Author
Muhammad Sohail
Language
English
Topic
Life Sciences / Molecular Biology, Life Sciences / Biochemistry, Life Sciences / Genetics & Genomics, Biochemistry, Science, Medical
Illustrator
Yes

Dimensions

Item Length
9.5in
Item Height
1in
Item Width
6.7in
Item Weight
31.8 Oz

Additional Product Features

Lc Classification Number
Qh450
Publication Name
Gene Silencing by Rna Interference : Technology and Application
Table of Content
Gene silencing by RNA interference and the role of small interfering RNAs. Basics of siRNA design and chemical synthesis. Oligonucleotide scanning arrays in the design of small interfering RNAs. siRNA production by in vitro transcription. Production of siRNAs with the application of deoxyribozymes. Production of siRNA in vitro by enzymatic digestion of double-stranded RNA. Plasmid-mediated intra cellular expression of siRNAs. Lentiviral vector-mediated delivery of si/shRNA. Exogenous siRNA delivery: protocols for optimising delivery to cells. RNAi in Drosophila cell cultures RNAi in Caenorhabditis elegans. Delivery of RNAi reagents in C. elegans by microinjection. RNAi in Xenopus laevis oocytes. RNAi in Xenopus embryos. An RNAi-based genomic library for forward genetics in the African trypanosome. RNAi in the malaria parasite Plasmodium. Silencing the expression of cysteine proteases (falcipains) in Plasmodium falciparum using RNA interference approaches. RNAi in transgenic animal models. RNA interference in mouse model. Virus-induced RNA silencing in plants. RNA silencing in plants - biolistic delivery of RNAi reagents. Agro-infiltration: a versatile tool for RNAi studies in plant.
Copyright Date
2005
Format
Hardcover
Lccn
2004-051934
Dewey Decimal
660.65
Intended Audience
Scholarly & Professional
Publication Year
2004
Type
Textbook
Dewey Edition
22
Number of Pages
400 Pages

Description de l'objet du vendeur

Booque

Booque

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4.7
Rapidité de l'expédition
4.7
Communication
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Évaluations comme vendeur (68)

o***m (174)- Évaluation laissée par l'acheteur.
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The Angkor book arrived finally, and is indeed beautiful. Thank you. The USPS tracking system is woefully inadequate. Tracking at one point indicated early delivery, which would have been great as this was a birthday present, but then got hung up at one facility for MANY days before moving onward to the destination. Arriving after the birthday. Tracking should NOT post an early arrival date and then have the package sit in limbo for days. DeJoy is killing the postal system.
j***0 (4719)- Évaluation laissée par l'acheteur.
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Beautiful new book, fast delivery. Very satisfied.
v***d (89)- Évaluation laissée par l'acheteur.
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Quick delivery and the product was as described. Thank you!

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